Rabbit liver 3-hydroxyhexobarbital dehydrogenase. Purification and properties.

3-Hydroxyhexobarbital dehydrogenase has been purified from the soluble fraction of rabbit liver. The enzyme gives the molecular weight of 42,000 by the method of sodium dodecyl sulfate-polyacrylamide gel disc electrophoresis and 33,000 by the Sephadex G-100 gel filtration method. Styrylmethylcarbinol, P-ionol, 1-indanol and I-tetralol are ako metabolized by the enzyme. Michaelis constants for 3-hydroxyhexobarbital are 5.3 X 10m4 M (with NAD+) and 4.0 X 10e4 M (with NADP+).